商务合作
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Business Model

ToloBio is eager to collaborate with innovative stakeholders across the CRISPR molecular diagnostics upstream and downstream industrial chain. We propose a model of project co-creation or joint development to shape the future together.


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Tolo Biotechnology is willing to create the future together with the innovative subjects in the upstream and downstream industrial chain of CRISPR molecular diagnostics in the mode of project co-creation or joint development.

Patent License


ToloBio initially identified the efficient "trans-cleavage activity" of the CRISPR-Cas12 protein. Leveraging this discovery, the company developed the HOLMES (one-HOur Low-cost Multipurpose highly Efficient System) nucleic acid rapid detection platform. In comparison to traditional molecular diagnostic technologies, CRISPR-Cas12-based detection technologies offer notable benefits, including high sensitivity, specificity, rapidity, and convenience. These attributes have led to their recognition as "the next-generation molecular diagnostic technology" by the journal "SCIENCE". Consequently, CRISPR-based diagnostic technologies hold great promise for applications in various fields such as pathogen detection, genetic disease screening, and precision medicine.

ToloBio holds methodological patents for Cas12-based nucleic acid detection. In 2020, ToloBio entered into an agreement with Sherlock Biosciences for a "Patent Cross-licensing" collaboration on Cas12 and Cas13 diagnostic technologies in the U.S. and China. Subsequently, in November 2022, ToloBio and Sherlock Biosciences expanded their collaboration by signing a global CRISPR diagnostic "Patent Cross-licensing" agreement. This agreement granted co-exclusive rights to Cas12 and Cas13 CRISPR diagnostic methods in markets outside of the U.S. and China.

Cas12 Series


AU2016278990B2

Novel CRISPR enzymes and systems

Novel CRISPR enzymes and systems

CN108513582B

新型CRISPR酶以及系统

新型CRISPR酶以及系统

CN109207477B

CRISPR酶以及系统

CRISPR酶以及系统

HK1220726A1

新的CRISPR酶和系統

新的CRISPR酶和系統

HK1253001A1

新型CRISPR酶以及系统

新型CRISPR酶以及系统

HK40011328A1

新型CRISPR酶以及系統

新型CRISPR酶以及系統

JP6898865B2

新規CRISPR酵素及び系

新規CRISPR酵素及び系

MX377162B

NUEVAS ENZIMAS Y SISTEMAS CRISPR

NUEVAS ENZIMAS Y SISTEMAS CRISPR

RU2737537C2

НОВЫЕ ФЕРМЕНТЫ И СИСТЕМЫ CRISPR

НОВЫЕ ФЕРМЕНТЫ И СИСТЕМЫ CRISPR

TWI758251B

新型CRISPR酶以及系統

新型CRISPR酶以及系統

US9790490B2

CRISPR enzymes and systems

CRISPR enzymes and systems

US10669540B2

CRISPR enzymes and systems

CRISPR enzymes and systems

US10648020B2

CRISPR enzymes and systems

CRISPR enzymes and systems

US11091798B2

CRISPR enzymes and systems

CRISPR enzymes and systems

CN110551800B

一种耐高温Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒

一种耐高温Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒

CA3102438C

Use of high-temperature-resistant cas protein, and method and reagent kit for detecting target nucleic acid molecule

Use of high-temperature-resistant cas protein, and method and reagent kit for detecting target nucleic acid molecule

CN108513582B

新型CRISPR酶以及系统

新型CRISPR酶以及系统

ZA202008068B

Use of high-temperature-resistant cas protein, and method and reagent kit for detecting target nucleic acid molecule

Use of high-temperature-resistant cas protein, and method and reagent kit for detecting target nucleic acid molecule

ZA202000682B

Application of cas protein, method for detecting target nucleic acid molecule and kit

Application of cas protein, method for detecting target nucleic acid molecule and kit

MOJ004548C

一種Cas蛋白的用途及靶標核酸分子的檢測方法和試劑盒。

一種Cas蛋白的用途及靶標核酸分子的檢測方法和試劑盒。

CN107488710B

一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒

一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒
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Cas13 Series


AU2017283713B2

Type VI CRISPR orthologs and systems

Type VI CRISPR orthologs and systems

NO3551753B1

CRISPR EFFECTOR SYSTEM BASED DIAGNOSTICS

CRISPR EFFECTOR SYSTEM BASED DIAGNOSTICS

KR102454284B1

신규 CAS13B 오르소로그 CRISPR 효소 및 시스템

신규 CAS13B 오르소로그 CRISPR 효소 및 시스템

US10266886B2

CRISPR effector system based diagnostics

CRISPR effector system based diagnostics

AU2016342038B2

Type VI-B CRISPR enzymes and systems

Type VI-B CRISPR enzymes and systems

AU2018234825B2

Novel CAS13B orthologues CRISPR enzymes and systems

Novel CAS13B orthologues CRISPR enzymes and systems

US10266887B2

CRISPR effector system based diagnostics

CRISPR effector system based diagnostics

KR102185464B1

신규 CAS13B 오르소로그 CRISPR 효소 및 시스템

신규 CAS13B 오르소로그 CRISPR 효소 및 시스템

EP3551753B1

Crispr effector system based diagnostics

Crispr effector system based diagnostics
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Other


CN216187183U

一种核酸样本存储装置

一种核酸样本存储装置

CN216192288U

一种电浸润微流控核酸扩增检测装置

一种电浸润微流控核酸扩增检测装置

CN215997965U

一种病毒保存管灭菌消毒装置

一种病毒保存管灭菌消毒装置

CN216005861U

一种断电续检的便携式等温扩增检测系统

一种断电续检的便携式等温扩增检测系统

CN215887004U

一种核酸自动提取装置

一种核酸自动提取装置

CN215884669U

一种病毒保存管存放架

一种病毒保存管存放架

CN215886978U

一种全自动核酸离心提取装置

一种全自动核酸离心提取装置

CN215887035U

一种等温核酸扩增系统装置安装架

一种等温核酸扩增系统装置安装架

CN215877989U

一种基于CRISPR技术的离心式微流控芯片

一种基于CRISPR技术的离心式微流控芯片

CN215878020U

一种荧光定量PCR检测用工作台

一种荧光定量PCR检测用工作台

CN215887006U

一种病毒运送保存管

一种病毒运送保存管

CN215887021U

一种实时荧光定量PCR检测系统

一种实时荧光定量PCR检测系统

CN215555339U

一种病毒保存管用存放箱

一种病毒保存管用存放箱

CN106319033B

一种检测染色体异常以及重组位点DNA序列的方法

一种检测染色体异常以及重组位点DNA序列的方法
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Technical Cooperation


01

HOLMES

Molecular Diagnostic Platform

02

HOLMES v2

Molecular Diagnostic Platform

03

SENA

COVID-19 Rapid Test

04

TB-QUICK

Tuberculosis Rapid Test

HOLMES

In 2017, ToloBio found that CRISPR-Cas12 protein has “trans-cleavage activity.” This led to the creation of HOLMES, a nucleic acid rapid detection method. HOLMES has vast applications like infectious disease detection, genetic disease screening, and home self-testing.

Business Cooperation

HOLMES

HOLMES v2

We've employed a thermostable CRISPR-Cas12b for a one-step system, merging isothermal amplification and CRISPR detection. This reduces contamination risk and streamlines the process, shortening testing time. This highlights HOLMES v2's potential for diverse molecular diagnostics.

 

Business Cooperation

HOLMES v2

SENA

ToloBio created SENA (Specific Enhancer for detection of PCR-amplified Nucleic Acids), a CRISPR-based technology, to address the “gray area” problem in qPCR. SENA rectifies incorrect diagnoses, enhancing accuracy and dependability.

Business Cooperation

SENA

TB-QUICK

Rapid, sensitive, and affordable diagnosis of M. tuberculosis is vital for controlling TB. ToloBio innovated a new TB DNA detection method, merging LAMP with CRISPR-Cas12b detection. This approach allows rapid and sensitive TB detection, offering a promising solution for TB diagnosis.

Business Cooperation

TB-QUICK

EXCLUSIVE TECHNOLOGY


Excellence

Efficient

Lean

Innovation

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Business Cooperation