92114 pET28TEV-OsCas12a

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92114 pET28TEV-OsCas12a

  • Specifications:
    • 1μg (20 μL, 50 ng/μL)

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Product overview


The pET28TEV-OsCas12a plasmid is an expression plasmid for OsCas12a (also known as OsCpf1) protein, which is used to express and prepare OsCas12a protein in Escherichia coli BL21 (DE3) strain. OsCas12a is derived from Oribacterium sp.NK2B42 strain. OsCas12a is a DNA endonuclease mediated by crRNA. When the target double-stranded DNA has a PAM (TTN) sequence, it specifically cuts the target double-stranded DNA, breaks the DNA double-strand and generates sticky ends. At the same time, when OsCas12a protein combines with crRNA and target double-stranded DNA to form a ternary complex, it will be activated for trans-cleavage activity against ssDNA, and the ssDNA in the system will be shredded. Therefore, the OsCas12a protein can be used not only for in vitro cleavage of target dsDNA, but also for the development of nucleic acid rapid detection kits, etc. The plasmid contains a lactose operon inducible expression system, which can be induced to express the target protein by IPTG or lactose and its analogues.



Storage Conditions

Store at 4℃; transport at ≤ 4℃.



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