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V32108E LbCas12a Powder

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V32108E LbCas12a Powder

  • Specifications:
    • 100 µg
    • 10×100 µg

Article number:

#V32108E


Price:Contact our distributors


隐藏域元素占位

Product overview

Description

LbCas12a (previously known as Cpf1) is a crRNA-guided DNA endonuclease derived from the Lachnospiraceae bacterium ND2006 strain. LbCas12a specifically cleaves the dsDNA target in a PAM (TTN)-dependent manner, generating DSBs with sticky ends, and cleaves ssDNA target in a PAM-independent manner. In addition, both dsDNA and ssDNA targets can trigger the trans-cleavage activity of LbCas12a, that is, when the LbCas12a binds crRNA and target DNA to form a ternary complex,the trans-cleavage activity of Cas12a will be released to non-specifically cleave ssDNA sequences in the reaction system.

This product is lyophilized glycerol-free LbCas12a nuclease, which can be shipped and stored at room temperature. After being dissolved with its reconstitution buffer, it shows the same performance as the non-lyophilized type of LbCas12a (Cpf1) Nuclease (#32108, ToloBio). This product can be provided in a variety of lyophilized forms such as powder and beads to meet the needs of different downstream applications

 

 

Components

LbCas12a Powder

V32108E-SP 

V32108E-01 

V32108E-02 

LbCas12a Lyophilized Powder (SCREW CAP TUBES)

10 μg 100 μg 100 μg × 5

Reconstitution Solution

200 μL 200 μL 1 mL

10 × HOLMES Buffer for Cas12a

1 mL 1 mL 1 mL × 5

 

 

Storage Conditions

This product is stored and shipped at room temperature. After reconstitution, the liquid enzyme should be stored at -20 °C or below and is stable for at least one month. Repeated freezing and thawing should be avoided for reconstituted liquid enzyme.

 

 

Experiment Procedure

LbCas12a (Cat# V32108E) CRISPR Trans-cleavage Experiment

1.Prepare the reaction mixture of CRISPR Trans-cleavage Experiment on ice as below.

Components

Volume 

Final concentration 

10 × HOLMES Buffer for Cas12a(#32042)

2 μL 1 ×

5 μM LbCas12a Nuclease

1 μL 250 nM

10 μM crRNA

0.5 μL 250 nM

1 μM Target DNA

0.5~5 μL 25~250 nM

10 μM HOLMES ssDNA Reporter(#31101)

0.5 μL 250 nM

Nuclease-free water

Up to 20 μL  

2.Put the reaction mixture in the real-time PCR instrument to detect the fluorescent signal at 37 °C, and collect the fluorescent signals every 30 sec.

 

 

Experimental Results

FAQ

Product Instruction

#V32108 LbCas12a (Cpf1) Nuclease (Lyophilized)

pdf

684.1KB

References

COA Query

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