V32108E LbCas12a Powder

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V32108E LbCas12a Powder

  • Specifications:
    • 100 µg
    • 10×100 µg

Article number:


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Product overview


LbCas12a (previously known as Cpf1) is a crRNA-guided DNA endonuclease derived from the Lachnospiraceae bacterium ND2006 strain. LbCas12a specifically cleaves the dsDNA target in a PAM (TTN)-dependent manner, generating DSBs with sticky ends, and cleaves ssDNA target in a PAM-independent manner. In addition, both dsDNA and ssDNA targets can trigger the trans-cleavage activity of LbCas12a, that is, when the LbCas12a binds crRNA and target DNA to form a ternary complex,the trans-cleavage activity of Cas12a will be released to non-specifically cleave ssDNA sequences in the reaction system.

This product is lyophilized glycerol-free LbCas12a nuclease, which can be shipped and stored at room temperature. After being dissolved with its reconstitution buffer, it shows the same performance as the non-lyophilized type of LbCas12a (Cpf1) Nuclease (#32108, ToloBio). This product can be provided in a variety of lyophilized forms such as powder and beads to meet the needs of different downstream applications




LbCas12a Powder




LbCas12a Lyophilized Powder (SCREW CAP TUBES)

10 μg 100 μg 100 μg × 5

Reconstitution Solution

200 μL 200 μL 1 mL

10 × HOLMES Buffer for Cas12a

1 mL 1 mL 1 mL × 5



Storage Conditions

This product is stored and shipped at room temperature. After reconstitution, the liquid enzyme should be stored at -20 °C or below and is stable for at least one month. Repeated freezing and thawing should be avoided for reconstituted liquid enzyme.



Experiment Procedure

LbCas12a (Cat# V32108E) CRISPR Trans-cleavage Experiment

1.Prepare the reaction mixture of CRISPR Trans-cleavage Experiment on ice as below.



Final concentration 

10 × HOLMES Buffer for Cas12a(#32042)

2 μL 1 ×

5 μM LbCas12a Nuclease

1 μL 250 nM

10 μM crRNA

0.5 μL 250 nM

1 μM Target DNA

0.5~5 μL 25~250 nM

10 μM HOLMES ssDNA Reporter(#31101)

0.5 μL 250 nM

Nuclease-free water

Up to 20 μL  

2.Put the reaction mixture in the real-time PCR instrument to detect the fluorescent signal at 37 °C, and collect the fluorescent signals every 30 sec.



Experimental Results


Product Instruction

#V32108 LbCas12a (Cpf1) Nuclease (Lyophilized)




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