V32108E LbCas12a Powder
V32108E LbCas12a Powder
-
Specifications：
- 100 µg
- 10×100 µg
Article number：
#V32108E
Price：Contact our distributors
隐藏域元素占位
Product overview
Description
LbCas12a (previously known as Cpf1) is a crRNA-guided DNA endonuclease derived from the Lachnospiraceae bacterium ND2006 strain. LbCas12a specifically cleaves the dsDNA target in a PAM (TTN)-dependent manner, generating DSBs with sticky ends, and cleaves ssDNA target in a PAM-independent manner. In addition, both dsDNA and ssDNA targets can trigger the trans-cleavage activity of LbCas12a, that is, when the LbCas12a binds crRNA and target DNA to form a ternary complex,the trans-cleavage activity of Cas12a will be released to non-specifically cleave ssDNA sequences in the reaction system.
This product is lyophilized glycerol-free LbCas12a nuclease, which can be shipped and stored at room temperature. After being dissolved with its reconstitution buffer, it shows the same performance as the non-lyophilized type of LbCas12a (Cpf1) Nuclease (#32108, ToloBio). This product can be provided in a variety of lyophilized forms such as powder and beads to meet the needs of different downstream applications
Components
|
LbCas12a Powder |
V32108E-SP |
V32108E-01 |
V32108E-02 |
|
LbCas12a Lyophilized Powder (SCREW CAP TUBES) |
10 μg | 100 μg | 100 μg × 5 |
|
Reconstitution Solution |
200 μL | 200 μL | 1 mL |
|
10 × HOLMES Buffer for Cas12a |
1 mL | 1 mL | 1 mL × 5 |
Storage Conditions
This product is stored and shipped at room temperature. After reconstitution, the liquid enzyme should be stored at -20 °C or below and is stable for at least one month. Repeated freezing and thawing should be avoided for reconstituted liquid enzyme.
Experiment Procedure
LbCas12a (Cat# V32108E) CRISPR Trans-cleavage Experiment
1.Prepare the reaction mixture of CRISPR Trans-cleavage Experiment on ice as below.
|
Components |
Volume |
Final concentration |
|
10 × HOLMES Buffer for Cas12a（#32042） |
2 μL | 1 × |
|
5 μM LbCas12a Nuclease |
1 μL | 250 nM |
|
10 μM crRNA |
0.5 μL | 250 nM |
|
1 μM Target DNA |
0.5~5 μL | 25~250 nM |
|
10 μM HOLMES ssDNA Reporter（#31101） |
0.5 μL | 250 nM |
|
Nuclease-free water |
Up to 20 μL |
2.Put the reaction mixture in the real-time PCR instrument to detect the fluorescent signal at 37 °C, and collect the fluorescent signals every 30 sec.
Experimental Results
FAQ
References
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